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CytoFLEX (B-R-V)

Blue-Red-Violet Series

The CytoFLEX Blue-Red-Violet (B-R-V) Flow Cytometer has the capacity for 15 parameters, including 13 for fluorescence detection. The fully activated instrument includes five channels from the 488 nm (Blue) laser, three from the 638 nm (Red) laser, and five from the 405 nm (Violet) laser.

The instrument includes 13 band pass filters which can be repositioned as needed.

  • 450/45
  • 525/40 (2)
  • 585/42
  • 610/20 (2)
  • 660/10 (2)
  • 690/50
  • 712/25
  • 780/60 (3)

Instruments with as few as four fluorescent channels activated are available with the ability to activate additional parameters as needed by purchasing an activation key. See the Products table below for a list of pre-set configurations or explore our online configuration tool.


Features

Harness the power of advanced sensitivity

Harness the power of advanced sensitivity Superior sensitivity and resolution across all channels give the CytoFLEX platform the edge over other cytometry systems four times its size. The graphs below demonstrate excellent resolution of all peaks using SPHERO™ Rainbow 8-peak beads.

488 nm Blue Laser Channels

CytoFLEX FITC channel 8-peak bead CytoFLEX PE channel 8-peak bead CytoFLEX ECD channel 8-peak bead CytoFLEX PC5.5 channel 8-peak bead CytoFLEX PC7 channel 8-peak bead

638 nm Red Laser Channels

CytoFLEX APC channel 8-peak bead CytoFLEX APC-A700 channel 8-peak bead CytoFLEX APC-A750 channel 8-peak bead

405 nm Violet Laser Channels

CytoFLEX Pacific Blue 450 channel 8-peak bead CytoFLEX Krome Orange 525 channel 8-peak bead CytoFLEX Violet 610 channel 8-peak bead CytoFLEX Violet 660 channel 8-peak bead CytoFLEX Violet 780 channel 8-peak bead
 

Specifications

Laser Specifications

Spatially Separated Laser Options (Beam Spot Size: 5 μm x 80 μm)

Laser Wavelength Power
Blue 488 nm 50 mW
Red 638 nm 50 mW
Violet 405 nm 80 mW
Flow Cell

Fixed integrated optics and quartz flow cell design with >1.3 numerical aperture. Flow Cell dimensions: 420 μm x 180 μm internal diameter

Forward Scatter Detection

Proprietary Axial Light Loss (ALL) sensor system using silicon photodiodes with built in 488/8 μm band pass filter.

Fluorescence and Side Scatter Detection

Fluorescence and side scatter light delivered by fiber optics to Avalanche Photo Diode detector arrays. Proprietary design ensures high performance, high efficiency, low-noise signal detection. Emission profiles are collected using reflective optics and single transmission band pass filters.

Violet Side Scatter Configuration

Option to configure Avalanche Photo Diode detector array to collect side scatter signal from Violet (405 nm) laser. The configured channel (VSSC) can be utilized to better resolve nanoparticles below 200 nm size threshold.

Quality Control

CytExpert QC automation pass/fail criteria rCV < 5.0% for the all 13 fluorescent channels.

PERFORMANCE

Blue (488 nm) Side Scatter Resolution

<300 nm

Violet (405 nm) Side Scatter Resolution (VSSC)

<200 nm

Forward and Side Scatter Resolution

Scatter performance is optimized for resolving human lymphocytes, monocytes, and granulocytes as well as nanoparticles.

Carryover

Single Tube Format: < 1.0%
Plate Loader Format: < 0.5%

Sensitivity

FITC: <30 molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: <10 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

The CytoFLEX Flow Cytometer is capable of achieving 3% rCV with alignment verification particles capable of rCVs <3%.

ELECTRONICS

Nominal Acquisition Rate

30,000 events per second with 15 parameters
Software capability to modify window extension parameter and to control abort rate during high event rate signal processing

Signal Processing

Fully digital system with 7 decade data display

Signal

Pulse area, height for every channel, width for one selectable channel

FLUIDICS

Ultra-low Pressure Peristaltic Sheath and Sample Delivery System

Low maintenance system
Sheath Pump tubing and Sample Pump tubing can be replaced by the user (no service visit required)

Sample Flow Rates

Fixed Flow Rates: 10, 30 and 60 μL/min
Custom Flow Rate Control mode from 10 to 240 μL/min in 1 μL increments.
Gravimetric calibration for absolute counts within CytExpert Software.

Fluid Capacity

Standard 4 L tanks
Optional 10L cubitainers

Automated Maintenance Functions

System Startup, Sample Mixing, Backflush, Prime, Shutdown, Deep Clean

Sample Input Formats

5 mL (12 x 75 mm) polystyrene and polypropylene tubes
1.5 mL and 2 mL microcentrifuge tubes

Plate Loader Formats

96-well Standard Flat, U and V bottom plates

DATA MANAGEMENT

Software

The CytExpert software is a full-feature software package that controls the instrument's operation, collection of experiment data, and analysis of the results.

Three different installation modes are available, depending on the level of security required. The Default installation requires no user login. For multiuser instruments, the User Management installation requires user login and contains features for role management. Electronic Records Management installation provides tools that facilitate compliance with 21 CFR Part 11, Electronic Records and Electronic Signatures.

An API (Application Programming Interface) is available and allows external software to perform operations such as running methods and for basic control of the plate loader.

Standardization

Daily QC beads or any other reference material that is relevant for your application may be used as the standardization sample to set target values and calibrate the gain settings automatically. The Standardization Target Library stores a variety of application target files which are linked to the reference material lot numbers.

Language

English and Chinese

Operating System

Windows® 7 Professional 64-bit
Windows® 8 Professional 64-bit
Windows® 10 Professional 64-bit

FCS Format

FCS 3.0

Minimum Specifications

CPU: Intel® I3 @ 2.9 GHz 1 Gigabit Ethernet port
RAM: 4 GB 2 USB 3.0 ports
Storage: 256 GB 4 USB 2.0 ports

Compensation

Automatic Full matrix compensation
Manual full matrix compensation
Novel Compensation Library: store fluorescent spillover values of dyes to easily determine the correct compensation matrix with new gain settings
Import/export compensation values between experiments
Absolute linear gain amplification enables the use of compensation settings between experiments and sample types

INSTALLATION

Dimensions (W x D x H)
Cytometer (with or without Plate Loader) Tanks and Holder
42.5 cm x 42.5 cm x 34 cm 14 cm x 35.6 cm x 35.6 cm
16.7 in x 16.7 in x 13.4 in 5.5 in x 14.0 in x 14.0 in
 
Weight

Cytometer: 23.4 kg / 51.6 lbs
Cytometer with Plate Loader: 28kg / 61.7 lbs

Power Specifications

Voltage: 100–240 V
Power: 150 -250 W

Operating Temperature Non-Condensing

15-27 °C, 59-80.6 °F

Accessories

For higher throughput applications, an optional plate loader module can save hands on time.

CytoFLEX Plateloader

Highlighted Features:

  • Plate Loader option can analyze a 96-well plate in as little as 32 minutes.
  • Option for integrated Sample Injection Mode Control, switch between single tube and plate acquisition in 5 minutes
  • Easy virtual plate layout setup with customizable wash and mix cycles
  • Define multiple experiments on a single plate
  • Compatible with flat-bottom, U- and V-bottom standard plates
  • The CytExpert API (Application Programming Interface) allows external software to run methods and control the plate loader using integrated automation.
 

Training Courses

File/Info Title Ref. Number
CytoFLEX Online Operator Training
CytoFLEX Online Operator Training

Videos

  • CytoFLEX: It Fits

    Click to play  

  • CytoFLEX: Powerful, Flexible, Easy

    Click to play  

  • CytoFLEX: Special Procedure

    Click to play  

  • Installing Your New CytoFLEX Plate Loader

    Click to play  

  • Unboxing Your New CytoFLEX Flow Cytometer

    Click to play  

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    488 nm

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    For Research Use Only. Not for use in diagnostic or therapeutic procedures.
    Class 1 Laser Product
    CytoFLEX will be available in selected geographies. Please check with your Beckman Coulter representative, for availability.
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