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Purification & Clean-Up

PCR Purification

  • PCR Purification

    PCR Purification

    Agencourt AMPure XP

    The Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats.

     

  • RNA & cDNA Clean Up

  • RNA & cDNA Clean Up

    RNA & cDNA Clean Up

    Agencourt RNAClean XP

    A simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions.

     

  • Sanger Sequencing Dye Terminator Removal

  • Sanger Sequencing Dye Terminator Removal

    Sanger Sequencing Dye Terminator Removal

    Agencourt CleanSEQ

    The Agencourt CleanSEQ system is a rapid, high performance dye-terminator removal process based on Solid Phase Reversible Immobilization (SPRI) technology. The paramagnetic bead format requires no centrifugation or filtration and is easily performed manually or fully automated for high throughput dye-terminator removal. The Agencourt CleanSEQ system produces sequences with longer Phred 20 read lengths and higher signal intensities than any other purification technology.

     

  • Size Selection

  • Size Selection

    Size Selection

    SPRIselect Reagent

    SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing. In this process, size selection is required to produce a uniform distribution of fragments around an average size. Using SPRIselect, the size distribution can be adjusted to suit the application and platform used. The process can be scaled for low to high throughput workflows.

    The guidance provided below can be used to optimize the desired size selection range. Used manually or automated on a liquid handling system such as the Biomek Laboratory Automation Workstation, SPRIselect will provide rapid and consistent size selection suitable for most applications.

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    RNACleanXP Certificate of Analysis
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